can
Wireless mice generally use batteries, which should be able to charge while using no relationship just like a wired mouse
One situation: When the notebook is charging, only the battery pack does not affect the other work of the computer, and the U port is not affected by the charger.
Another situation: When using U port to do multiple work access, such as using U port 1 to charge the mobile phone at the same time, using U port 2 to read the U disk, using U port 3 to insert the hard disk box, using U port 4.5 to access the U port keyboard and mouse.... ... . Will not affect the U port's respective work.
You can play with the mouse. It doesn't matter.