Disinfection pot biological detection is usually divided into the following steps:
1. Sample preparation: Select the disinfection pot sample to be tested, which can be the brush, gauze and other items in the disinfection pot, or the dust and dirt remaining in the pot. Place the sample in a container, taking care to avoid cross contamination.
2. DNA extraction: DNA extraction kit is used to extract DNA from the sample. The extraction method is carried out in accordance with the kit instructions, generally including cell rupture, protein digestion, DNA precipitation and other steps.
3. Amplified DNA: The extracted DNA is amplified by PCR technology. Appropriate primers and PCR conditions were selected according to specific detection requirements, and the extracted DNA was mixed with primers and PCR reagents for PCR reaction. 4. Preparation for electrophoresis: The amplified PCR product is mixed with DNA markers and added to the agarose gel hole. 5. Electrophoresis: Put the agarose gel plate into the electrophoresis apparatus, set the appropriate electrophoresis conditions, such as current intensity, electrophoresis time, etc., for electrophoresis.
6. Visualization results: According to the migration of DNA bands in agarose gel plates, the results of amplified products can be visualized by ultraviolet irradiation or other staining methods. 7. Result analysis: DNA bands on agarose gel can be observed to analyze whether there are target organisms according to the size and quantity of PCR products.
It should be noted that the specific steps of biological detection of disinfection pot and the use of reagents may vary due to different experimental purposes and detection methods, and the above steps are for general reference only. When performing biological tests, it is recommended to follow professional instructions and operating instructions for experiments.