First, in principle, the Illumina sequencing chip is a bridge amplification, forming a \"cluster\
Secondly, in terms of performance, the throughput of Illumina sequencers is extremely large, and the current HiSeq X has reached 1.6-1.8T; 454 is the longest reading length (these three); Because of the characteristics of ABI, its accuracy should be the highest. At the end of the day, Illumina is expensive, especially for late-stage, high-throughput sequencers that are not affordable in ordinary laboratories.
At the same time, their instruments may be supervised, designated such instruments can only carry out sequencing of a certain biological sample, of course, in fact, the cost is still in; The disadvantage of 454, only compared with these three, is that its homopolymer accuracy is low; For SOLiD, that is, the reading length is particularly short, and because of the sequencing principle of SOLiD, the sequencing system is relatively complex, so the SOLiD system is not good to upgrade, and it seems that the SOLiD system has rarely been heard of at present.