ChromiumTM Single Cell 3 'Solution is a technology based on the 10 X Genomics platform that can isolate, label, and test 500-10,000 single cells at the single-cell level at a time through a microfluidic system. Rapid distribution of single cells or long fragments of DNA molecules into water-in-oil microreaction systems. The single DNA molecules in each microreaction system will receive unique molecular labels. The sequencing library generated can be sequenced on illumina platform, and the data analysis and visualization software provided by 10x Genomics. Sequencing experiments and analysis processes can be seamlessly integrated with illumina systems. The platform is widely used in 3 'single cell transcriptome sequencing, genome assembly applications, immune pool sequencing, ATAC-seq, human whole genome and whole exome sequencing.
10x Genomics single-cell transcriptome sequencing effectively solves the difficult problem of cell heterogeneity that tissue samples cannot crack, and helps to discover new cell types. Based on a large number of single-cell gene expression data, it can provide cell expression feature clustering, subgroup expression feature analysis, marker screening and other aspects of analysis, which is an efficient single-cell gene expression level detection technology.
Advantages and disadvantages of 10X GENOMICS single-cell transcriptome sequencing
Advantages of 10x Genomics single cell transcriptome sequencing
1, simple and convenient: single cell sorting, expansion, library building in one;
2, high cell flux: the number of cells per sample can reach 500-10000;
3. Short library building cycle: 1 day can complete cell suspension preparation, single cell capture, expansion and library building;
4, ultra-high capture efficiency: single cell capture efficiency up to 65%;
5. True single cell: The probability of a single drop capturing multiple cells is very low (0.9%\/1000cells);
6, affordable: Compared with other single-cell platforms, affordable.
Drawbacks of 10x Genomics single cell transcriptome sequencing
1. Non-full-length information: only 3 'end transcript information can be obtained;
2, the sample requirements are high: the starting amount of a single sample of cells is 10^5-10^6, the number of living cells needs to be more than 80%, it is recommended that more than 90% is the best.
10X genomics is a high-throughput genomics and single-cell analysis technology. Its main feature is to divide the DNA molecules of a single cell into strip molecules, add unique barcode sequences and chip chips for amplification, and then obtain the genomic information of the segmsegmed strip molecules of each cell through high-throughput sequencing. This enables single-cell analysis and high-resolution genome sequencing.
This technique has the advantages of high coverage, high sensitivity and high accuracy, and can be applied to complex cell systems and fine expression analysis.