Naturally, DNA polymerase penetrates a nucleotide into a DNA molecule and releases an H, resulting in a locally detectable PH change. If binding occurs, H is released and the PH of the corresponding solution changes, which is detected by the ion sensor and converted into a digital signal.
If there are two identical bases on the detected DNA strand, the detected voltage doubles, and the chip records two identical bases.
If the next nucleotide reached by the template does not match the nucleotide in the microchip stream, the voltage is not detected and the bases are not recorded.