The gene chip is the transcriptome.
The nucleic acid sequence was determined by hybridization with a group of nucleic acid probes with known sequence, and a probe with known sequence of target nucleotides was fixed on a substrate surface. When the nucleic acid sequence TATGCAATCTAG with fluorescent labeling in solution is complementary matched with the nucleic acid probe at the corresponding position on the gene chip, a set of completely complementary probe sequences are obtained by determining the position of the probe with the strongest fluorescence intensity. The target nucleic acid sequence can be reassembled accordingly.