Protein chip is a high-throughput protein function analysis technology, which can be used for protein expression profiling, studying protein-protein interaction, even DNA-protein, RNA-protein interaction, screening protein targets for drug action.
principle
The research object of protein chip technology is protein. Its principle is to carry out special chemical treatment on solid phase carrier, and then fix known protein molecular products on it (such as enzymes, antigens, antibodies, receptors, ligands, cytokines, etc.). According to the characteristics of these biomolecules, Capture the protein to be tested (existing in serum, plasma, lymph, interstitial fluid, urine, exudate, cytolytic fluid, secretory fluid, etc.), wash, purify, and then confirm and biochemical analysis; It is used to obtain important life information (such as unknown protein components, sequences). The biological function of expression level in vivo, the mutual regulatory relationship with other molecules, drug screening, drug target selection, etc.) provides strong technical support.