Gene chip is also called DNADNA microarrays can be divided into three main types: 1) nucleic acid probes or cDNA fragments fixed on the surface of a polymer substrate (nylon membrane, nitrate fiber membrane, etc.), usually hybridized with isotopically labeled target genes, and detected by radiography. The advantage of this method is that the required detection equipment is consistent with the current radiography technology used in molecular biology and is relatively mature.
The biochips commonly used in clinical biochemistry are mainly genearrays and proteinarrays.Also known as proteinmicroarrays (proteinmicroarrays) and laboratory on a chip three categories.
There are three main types of protein chips: protein microarrays, microporous plate protein chips, three-dimensional gel block chips, etc. Protein microarrays Macbeath and SchreiberL et al., Harvard University, reported the study of making protein chips by means of a spot-like mechanical device, which immersed the tip of a needle into a microhole containing a purified protein solution, then moved it to a slide, and sprayed 1nl of the solution on the surface of the slide, and then the manipulator repeated the operation to spot different proteins.
ymetrix Corporation. The company has a collection of computer, mathematical and molecular biology experts, its annual research funding of more than $10 million, has been around for six or seven years, and has a number of patents. The products are about to or have been partially put on the market, and the social and economic benefits generated are impressive.3, there will not be only one DNA molecule on the gene chip, in order to improve efficiency and save costs, there are generally multiple DNA sequences if multiple points on the chip color, indicating that there are multiple target sequences in the sample, because the DNA sequence on the chip is known, so according to the principle of complementarity, it can be knownSequence in a sample. Same as 2, the sequence is known, that is, when you make the chip, you make it with the DNA of the known sequence.
4, the method combines the microelectronic lithography technology with DNA chemical synthesis technology, can greatly improve the probe density of gene chips, reduce the amount of reagents, and achieve standardization and mass production, which has a very important development potential.
1. Domestic chip brands include ZTE, Hisilicon, Sunway, Feiteng, SMIC and so on.
2, what are China's domestic chips China's top ten semiconductor companies are: Weil shares, Unigroup Zhanrui, Yangtze River storage, ZTE micro, Hisilicon, Zhaoyi Innovation, Mullinsen, Geke Micro, Shilanwei, Goer shares. Weil is one of the world's top three CMOS image sensor chip design enterprises and the world's second largest automotive CIS manufacturer, founded in 2007, headquartered in Shanghai.
3, the domestic chip brands are ZTE, Hisilicon, Sunway, Feiteng, SMIC. Zte chips have reached the international level in terms of technical capabilities, with front-end design, back-end design toThe whole process customization capability of packaging and testing, its complex SoC chip design capability has reached the international leading level, with the whole process customization capability from front-end design, back-end design to packaging and testing, can provide the overall chip solution.